Weekly Reflection for Week of 03/25/16

Finicky Flies and Sex

Yes, I wrote “sex” in my title.  Get over it.  Because I am not talking about what you’re probably thinking about…well I mean you have to have that before you get the sex I’m talking about, but…oh never mind!  The reason I bothered mentioning it was because not all genes follow autosomal recessive inheritance patterns.  In truth, there are  a variety of ways that traits can be inherited in offspring.  One of these ways is that the alleles for the gene are inherited on the XY sex chromosomes of an organism.  And this week, we looked at how sex-linked inheritance works via the bountiful fruit flies.  As such, I’d call this a Science Practice numero Seis week.  (Click here, no, wait, HERE to find out what that means!)

yucky
It’s…so…yellow.

So right now we are muddling ourselves through our genetics unit.  This week we used a sorta-kinda-lab-practical online that was VERY VERY AWESOME to trace geno- and phenotypes in fruit fly families to determine the modes of inheritance various traits use.  I found this very entertaining and started to recognize the pattern of autosomal recessive inheritance based off of phenotypes in the F1 generation.  Even the sex-linkage didn’t throw me too much of a problem; no, it was when I tried to determine the mode of inheritance for body color that really threw me.  The chi-square tests I made for both autosomal recessive and sex-linkage both failed, and by that time I was too exhausted to try another one.  It bothers me to this day that I couldn’t figure that one out…

The good news in this is that the lab served as a benchmark for my chi-square skills.  I feel much more confident than I did before in using the test and various tables associated with it. e, o, e-o, (e-o)², (e-o)²/e, DF, and x² value for comparison!  WHEEEEEEEEEE I FEEL SMAAAHHHHHHHHRTTTTTTT!!!!!!!  The bad news is that 1. I missed two days of class due to a competition I participated in WHICH I DON’T EVEN KNOW THE RESULTS OF and 2. In those two days I missed a quiz which would have further served as a measurement for me to get a feel for how I am doing in this unit academically.  I feel self-assured in my skills, but there’s this nagging thought that I haven’t fully grasped each and every concept, which could really hurt me come AP Biology test time, which is in T-42 days, YIKES!  So moving forward in the next month what I really want to do is to refine and hammer in those skills and the information that I know I’ll be needing to get that perfect “5” on that test…not that I wouldn’t mind taking another year of Biology, haha! 😉  I suppose that I’ll have to do some extra work, such as the practice tests, and this really neat app called “Varsity Tutors” which recreates sample questions from pretty much any standardized test out there.

So that’s it for this go-around.  Here’s a question for you as a wrap up; what are your favorite study habits?  Are you a night-owl with your study hours or a morning bird?  Comment below as you please!

Permanezcan  homeostático,

Jess

Weekly Reflection for Week of 03/18/16

Mendel, Mendel, Mendel, you Crazy, (Lying?), Monk

Buenos tardes my bio pals!  How goes it?  On this end, it’s a bit crazy between college decisions, scholarship work, national tests, extracurriculars, prom,  oh, and a little thing I like to call school.  Because that somehow manages to exist still.  So.

This week it was the week of Mendel.  Well, actually, his work and theorems, really, because who cares about a monk who contributed greatly to the way we predict genotypes and phenotypes of children even though he sorta-kinda-maybe-probably faked all his data but it was sound enough.  You follow?  (If not, click here!)

confused_scifi

For the majority of the week we were focused on learning and applying good ol’ Mendelian mathematics to different genealogy problems, (ie-predicting the chance of offspring traits based from parents), which makes this a Numero Dos and Seis week.  Good math and using and working with explanations and theories, to simplify it for you. PunnettSquare The two go hand-in-hand with Mendel, who predicted the traits of pea plants with his now famous Punnett Squares.  This was exemplified by the BioInteractive packet we completed early-ish in the four day week we had.
My favorite parts of it were when we had to apply the previous information we learned about sickle cell anemia and malaria to our analyses of our Chi-Square Analyses in order to make sense of the data we got as well as when we had to determine which children were likely to have malaria or colorblindness due to the phylogeny tree provided.  Even better was when I got to create my own phylogeny tree for the described families!

The hardest part of this week for me was finishing up the Polymerase Chain Reaction laboratory write-up, mostly in the Chi Square Analysis.  When creating it, I had thought that I had the correct predicted/expected values for my class and Pushtoon, (Afghani), populations.  But when I proudly carried my 14 page lab into class and asked my teacher to just quickly look at the Chi Squares to check for accuracy, my little castle of perfection just kind of crumbled over.  Which really did stink.

The good thing is that I looked over the work and saw where I went wrong exactly.  Additionally, I believe that I have gotten a strong handle over the Mendelian mathematics because the first sheet I did today, (Monday), I felt confident and in control of the math I had to use in order to get the right answers to the questions.  Overall, it’s a much better position than what I felt I was in last week.

There’s not much more I think you’d like me to detail you in on.  Thanks for reading, and enjoy the slowly but surely Spring coming!  Until next time!

Permanezcan homeostático,

Jess

 

Weekly Reflection for Week of 03/11/16

Biotechnology Rules!

Really, it does.  Albeit I am partial; I’ll expand on that in a bit.  And I know, I know, it’s Tuesday!  But take DST to the extreme, (ahem, 24 hrs-ish), and you’ll find that I’m so on time.

This week we had what I’m pretty sure will go down as the lab of the year for AP Bio-PCR amplification.  Not only did we get to see samples of DNA run down through an agarose gel during electrophoresis, but we also analyzed it.  This may seem kind of exciting but also somewhat boring except one itsy, bitsy, key detail.

The genetic samples were our own.

That’s right, I got to analyze my own DNA to see whether or not I had a certain gene.  (Turns out I don’t have it-pooey).  And because of this, I’m going to call this week a Science Practice numero Tres, Cuatro, Cinco, and Siete.

So, numero tres was accomplished by asking a VERY scientific question;

What is the frequency of the alu insertion gene in the AP Biology class?

Check.  Done.  Move on, because this question was the motivation factor for EVERYTHING this week.

Numero Cuatro was the absolute biggest portion and scientific practice of the week.  During our lab we had to collect the data by performing a procedure with no mistakes, or else the data would be skewed.  And that would really stink considering we don’t have wiggle room for extra runs.  That being said, I have to toot my own horn.  I felt very confident and well-practiced with the lab.
woman_microbiologist.jpgI was worried the night before our first day that my hands would shake too much, or that I would mislabel or confuse samples, but I didn’t.  In fact, I kind of led my lab station through the process, a situation I definitely didn’t find myself too often in my Sophomore year of Biology.  I helped build confidence in my classmates with their abilities to use the Biotech, and in doing so I built up my own confidence.  It’s really cool, actually, to experience something like that.  But don’t be mistaken-I WILL NEVER be a high school teacher if I can avoid it.

Numero cinco was accomplished once our DNA bands appeared in the agarose gels.  From here we were able to determine whether or not we had the alu insertion in our DNA.  I was really glad that my lab station partner in crime was with me because the lab station across  of us had a DNA sample that didn’t show up, and I claimed that it meant that the person who the sample came from didn’t have the insertion.feud_x.jpg  If not for my partner, I would have incorrectly maintained this assumption, and potentially have influenced others into thinking the same.  After this, we analyzed the data, (correctly!), and found that neither one of us had the insertion.  One of our station mates, however, did, and he was one of the two homozygous dominant samples in the class population.  ¡Que incredíble!

Moving forward I hope that in our Mendel genetics unit I will be as successful in the math as I was with handling the biotechnology in this lab.  In truth, it will take a careful eye and self-encouragement, two things that in general I could work on.  Well, I suppose that’s it for this week!  Until next time!

Permanezan homeostático,

Jess

Weekly Reflection for Week of 03/04/16

Splitting up on Cancer

Glad to see you back!  (Well, I guess I can’t exactly see you, but, oh well!)  I don’t have much pop culture to reference to or cite tonight, so let’s jump right in, shall we?

This week hit home for me.  cell-cycleIn class we did a lot of work with cell division, particularly with how the cell cycle, (see photo), works and potentially can go wrong.  The majority of the time when something goes wrong in the cell cycle it’s due to a regulation gene being mutated.  So cells may not duplicate enough genes, may not create enough organelles, may not grow enough, or may grow too fast without regulation.  The final piece, my dears, is what emotionally hit me.  Quite a few members of my family have had cancer, and by learning what was going on in their cells fascinated me.  And that is what made this week a Science Practice numero Seis and Siete week!  AKA-working with explanations and theories while connecting domains of knowledge.

The piece of work that I’d like to focus on today is the packet about cancer.  While completing the packet we worked through a click-‘n-learn activity from BioInteractive, (a FABULOUS resource!), to aid in our understanding of the material with videos and easy-to-comprehend materials.BRCA1_de.png  My favorite part of this was how the terminology wasn’t made over-simplistic to understand, which some materials do try and use as a tool for comprehension.
Instead, this used the proper terminology and effective graphics in order to explain how the two different genes affect tumor growth.  Now I know how BRCA1 and P53 genes work!  Additionally, the packet showed how this mutation in the DNA can affect the organism as a whole as the cells grow, become cancerous, and migrate to other parts of the body.  There’s even a whole industry dedicated to helping men and women work through their cancer experiences.

Moving forward I want to make sure I understand the checkpoints of each part of the cell cycle and can properly keep everything in order.  G1, S, G2, Mitosis/Cytokinesis, which then further divides into PPMAT, (Prophase, Pro-Metaphase, Metaphase, Anaphase, and Telophase!)  My biggest difficulty is looking at cells under a microscope and positively ID-ing which cells are in which phase, particularly P, P, and M, because many times the cells kind of look the same to me, save for anaphase and early to mid-telophase.  That’s all for now, folk!  Until next time,

Permanezan homeostático,

Jess

 

Weekly Reflection for Week of 02/26/16

Genetics and Jeff

Hey hey hey!  Glad to see that you’re up and reading after the Oscars, (so much Mad Max, am I right???  At least Spotlight shone in the end!).  So before I go all geeky and rage against Star Wars not winning one. Single. Oscar. I’ll STARt up on this post!

Alright, so this week we officially got into the deep end with our genetics unit.  We covered Sciences Practices Uño, Dos, Cinco, and Seis.  (If you need a refresher on what’s what, click HERE).  And this was accomplished through two activities, one of which I’d say was rather important; our argument about Jeff!

jeff
This is Jeff

So Jeff M. has a theory that he is related to Mrs. H, Mr. H, and the three little H’s.  Unfortunately, apparently mitochondrial DNA tracking doesn’t exist for some reason, and Mr. H is dead, so only Mrs. H and her three youngins’ are the only sources of comparable genetic material.  Basically, based upon the genetic markers taken from each individual and the position of the markers, you had to decide whether or not this guy’s story was for real.  So…that knocks out Uño, Dos,  Cinco, and Seis.  Jeff had a THEORY, (Seis), that he was related to this family, (who knows, maybe he wants some stowed-away money from them?), that you had to determine was true or not by ANALYZING the given data, (Cinco).  Once you came to your conclusion, you had to support your own conclusion with an explanation, (Seis), backed with good use of the data, and, (if you want even better science, and you ALWAYS do!), a mathematical test to prove even more so that you are correct, (Uño).  Personally, our argument fell flat because we didn’t analyze all of the possibilities that the data and model showed and we didn’t perform a statistical analysis which could have shown us that Jeff wasn’t related.

I enjoyed learning about the hard science behind the activity, but I know that I couldn’t do this for a living.  Firstly, I failed to understand at first why Jeff may NOT be related to the H family, (ONE MARKER MAKES ALL THE DIFFERENCE!), and I also didn’t care too much about how the family would deal with this information.  Which definitely tells 142054611a87831c250c3e0dfedc0dffme that I couldn’t play genetic counselor or genologist.  It was nice, however, to see the other side of the argument that Jeff isn’t related because it helped me look at the problem from another viewpoint, as well as reinforce understanding of the concept we were trying to learn.  I now can see the correlation between density of the marker and position on a chart more clearly, and how that contributes to a family’s genetic tree.
Moving forward I would like to improve my mathematical skills to help make my explanations and theories become more logical and sensible.  I’m sure I’ll have plenty of opportunity for this as we continue into the human genome unit, especially once we get into Punnett Squares, genotypes, and phenotypes of populations.

Well, that’s all for this week folks.  Until next time!

Permanezan homeostático,

Jess